Phosphotungstic Acid, Water, and Histologic Stains
نویسندگان
چکیده
منابع مشابه
Hot Alcoholic Phosphotungstic Acid and Uranyl Acetate as Routine Stains for Thick and Thin Sections
One of the potential advantages of high-voltage electron microscopes is that their greater penetrating power allows the use of thick sections (0.22 lt) to study three-dimensional cytoarchitecture . So far, this advantage has been limited because conventional methods of staining only allow the penetration of heavy metal solutions into sections to a depth of a few hundred Angstroms . For example,...
متن کاملSection 8.1 - Nucleic Acid Stains
Molecular Probes prepares the most extensive assortment of nucleic acid stains commercially available, many of which have been developed in our research laboratories. This section discusses the physical properties of the various classes of dyes, listed below. The sections in Chapter 8 that follow discuss numerous applications of these dyes and our other reagents and technology for genomics rese...
متن کاملThe Reactivity and Staining of Tissue Proteins with Phosphotungstic Acid
After aldehyde-fixation, treatment with phosphotungstic acid (PTA) in aqueous acidic medium was shown to produce an intense electron-opaque stain with minimal distortion of organelles. Mitochondrial matrix, cisternae of the endoplasmic reticulum, and the Z-band of muscle were densely stained, whereas membranes stood out in negative contrast. Staining of glycogen or lipid was not apparent. Under...
متن کاملPhotocatalytic Decolorization of Methyl Orange Solution with Phosphotungstic Acid
Photocatalytic decolorization of Methyl Orange (MO) solution has been studied using phosphotungstic acid (HPA) as photocatalyst. The decolorization reaction of MO solution is photocatalysis. The paper reveals that the optimal loading of photocatalyst for decolorization of MO solution (10 mg/L) is 0.6 g/L. The results show that the photocatalytic decolorization reaction of MO with HPA in a h...
متن کاملThe Photometric Determination of Cystine , Cysteine , Ascorbic Acid , and Related Com - Pounds with Phosphotungstic Acid
Some time ago a brief report (1) was made on the use of the Pulfrich photometer for the determination of sulfhydryl and disulfide compounds by a modified Folin (2) method. Since then the method has been extensively used in this laboratory (3-9). Folin’s method is based upon the observation that cystine plus sulfite gives a blue color with phospho-18-tungstic acid; whereas cystine alone develops...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1999
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500064518